RNA

Part:BBa_K2246005

Designed by: JUAN CARLOS RUEDA SILVA   Group: iGEM17_TecCEM   (2017-10-02)


SOD siRNA

siRNA sequence for silencing superoxide dismutase [Cu-Zn] (SOD) gene in Diaphorina Citri. This sequence must be used as dsRNA, in this way it works as a small interfering RNA specific for superoxide dismutase [Cu-Zn] (SOD) gene in D.citri. Cell metabolism CSD1 is an important superoxide dismutase involved in detoxification of superoxide radicals, therefore it silencing result in an increase of mortality rate (Taning, Andrade, Hunter, Christiaens & Smagghe, 2016). The selected target sequence, in order to maximize specificity, was choosen by comparing the sequence of this gene against several species, and an exclusive region from D.citri was selected. Then a BLAST was run in order to eliminate the possibility of hybridization with other species.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None