Part:BBa_K2246004

WNT siRNA

siRNA sequence for silencing protein wingless-like (WNT) gene in Diaphorina Citri. This sequence must be used as dsRNA, in this way it works as a small interfering RNA specific for wingless-like (WNT) gene in D.citri. Involved in wing and nymph development. The down expression of this gene leads to wingless psyllids, inducig mortality and affecting maturing (Vyas, Fisher, He, Nelson, Yin, Cicero, Willer, Kim, Kramer, May, Crow, Soderlund, Gang & Brown, 2015). The selected target sequence, in order to maximize specificity, was choosen by comparing the sequence of this gene against several species, and an exclusive region from D.citri was selected. Then a BLAST was run in order to eliminate the possibility of hybridization with other species. Vyas M, Fisher TW, He R, Nelson W, Yin G, Cicero JM, et al. (2015) Asian Citrus Psyllid Expression Profiles Suggest Candidatus Liberibacter Asiaticus-Mediated Alteration of Adult Nutrition and Metabolism, and of Nymphal Development and Immunity. PLoS ONE 10(6): e0130328. doi:10.1371/ journal.pone.0130328

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]