Part:BBa_K2246003
RacI siRNA
siRNA sequence for silencing rac GTPase-activating protein 1 (RacI) gene in Diaphorina Citri. This sequence must be used as dsRNA, in this way it works as a small interfering RNA specific for rac GTPase-activating protein 1 (RacI) gene in D.citri. This gene encodes a GTPase-activating protein (GAP) that is a component of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Among its related pathways are Immune System and Vesicle-mediated transport. In other insects attacked by parasitoids and pathogens, Rac1 up-regulation has been linked to hemocyte recruitment. In D.citri this gene is up-regulted, which suggest the overexpression affects the efective inmune response againts Candidatus liberibacter asiaticus (Vyas, Fisher, He, Nelson, Yin, Cicero, Willer, Kim, Kramer, May, Crow, Soderlund, Gang & Brown, 2015). The selected target sequence, in order to maximize specificity, was choosen by comparing the sequence of this gene against several species, and an exclusive region from D.citri was selected. Then a BLAST was run in order to eliminate the possibility of hybridization with other species.
Vyas M, Fisher TW, He R, Nelson W, Yin G, Cicero JM, et al. (2015) Asian Citrus Psyllid Expression Profiles Suggest Candidatus Liberibacter Asiaticus-Mediated Alteration of Adult Nutrition and Metabolism, and of Nymphal Development and Immunity. PLoS ONE 10(6): e0130328. doi:10.1371/ journal.pone.0130328
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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