RNA
Part:BBa_K206030
Designed by: Amelia Hardjasa and Alex Ng Group: iGEM09_British_Columbia (2009-05-11)
Lock 1
crR12-loop-RBS sequence taken from Isaacs et al. (1), with modifications to remove an extra EcoRI site. crR12 binds to the RBS, preventing translation of the mRNA unless a matching "key" RNA is also present in the cell.
1) Isaacs, FJ, Dwyer, DJ, Ding, C, Pervouchine, DD, Cantor, CR, and Collins, JJ. Engineered riboregulators enable post-transcriptional control of gene expression. 2004. Nature Biotechnology 22(7):841-847.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |