Translational_Unit

Part:BBa_K2029013

Designed by: Bui Hoang Duy   Group: iGEM16_UBonn_HBRS   (2016-10-14)

nprb_EstC2

The carboxylesterase (EstC2) is a catalytic enzyme preceded by secretion tag pP43_RBS_nprb. The used prefix pP43_RBS_nprb allows the secretion of EstC2 in B. subtilis.

Usage and Biology

This part can be used to produce and secrete EstC2 in B. subtilis. EstC2 can hydrolyse ester molecules into it's alcohol and acid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 381
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 333
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None