Regulatory

Part:BBa_K1994019

Designed by: Liam Carroll   Group: iGEM16_Warwick   (2016-09-26)


Multiple dCas9 binding site sequence

Promoter sequence containing multiple PAMs staggered at regular intervals to provide multiple possible sites for dCas9 binding.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 225
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 229
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/crispr
Parameters
None