RNA

Part:BBa_K199038

Designed by: Romina Clemente   Group: iGEM09_MoWestern_Davidson   (2009-07-14)

pBad-CUAGU Suppressor tRNA

Causes low expression of a suppressor tRNA that suppresses a 5-bp codon CGGUC, Part K199028. Update: these tRNA parts do not work as expected, but instead appear to read four-base codons. See [http://www.ibc7.org/article/journal_v.php?sid=287]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 139
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 176
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/apBad-CUAGU Suppressor tRNA