Part:BBa_K1974020

Snowdrop lectin+linker+6X His-Tag

Introduction:

Figure 1.snowdrop lectin+linker+6xHistag

      Snowdrop (Galanthus nivalis) lectin is a protein that has special affinity to the glycoprotein on the surface of insect epithelial cells. According to reference, snowdrop-lectin is resistant to high temperature and would not be degraded by digestive juice. The species-specificity is based on the toxin, and the snowdrop lectin is the role of the carrier. When it is ligated by Pantide, which can increase the oral activity of Pantide.

Figure 2.. Snowdrop lection The DNA sequence length of Snowdrop lectin is around 300-400 b.p. In this PCR experiment, the product’s size should be close to 550-700 b.p.

Figure 3.

Figure 4.

Figure 5.

Figure 6. Mechanism: In the alimentary canal, lectin binds to gut epithelial glycoproteins, and crosses the epithelium cell by endocytosis and exocytosis, and transported into haemolymph.

Reference

1. Elaine Fitches, Martin G. Edwards, Christopher Mee, Eugene Grishin, Angharad M. R. Gatehouse, John P. Edwards, John A. Gatehouse “Fusion proteins containing insect-specific toxins as pest control agents: snowdrop lectin delivers fused insecticidal spider venom toxin to insect haemolymph following oral ingestion,” Journal of Insect Physiology, 2004, 50, pp.61-71
2. Elaine C. Fitches, Prashant Pyati, Glenn F. King, John A. Gatehouse, “ Fusion to Snowdrop Lectin Magnifies the Oral Activity of Insecticidal Omega-Hexatoxin-Hv1a Peptide by Enabling Its Delivery to the Central Nervous System,”

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]