Part:BBa_K1929300
Interleukin-2 (IL-2) with designed RBS
Interleukin-2 (IL-2) protein coding sequence. Silent point mutation at base pair insert 272 (A>T) to make BioBrick compatible (removed an XbaI site). Designed RBS with spacer DNA added preceding the IL-2 for protein expression.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 630
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
MIT_MAHE 2020
Summary
Interleukin-2 (IL-2) is an important immunological signal that influences various activities like differentiation, immuno responses and homeostasis. Stimulation with IL-2 is crucial for the maintenance of regulatory T cells and the differentiation of CD4+ T cells into defined effector T cell subsets following antigen-mediated activation. It is presented either in a soluble form or bound to dendritic cells and the extracellular matrix. It can with or without complexing with IL-2 specific antibodies amplify CD8+ T cell responses and helps in the upregulation of Treg cell population. Thus, they are involved in immune stimulation or suppression activities.
References
1. Boyman, O., Sprent, J. The role of interleukin-2 during homeostasis and activation of the immune system. Nat Rev Immunol 12, 180–190 (2012). https://doi.org/10.1038/nri3156
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