RNA

Part:BBa_K1828900

Designed by: Altynay Abdirakhmanova   Group: iGEM15_NU_Kazakhstan   (2015-09-09)


Ptet+sgRNA for ori

This composite part was designed to be a part of Blue Light Safety system that targets origin of replication of pMSP3535 plasmid. This plasmid works in gram positive bacteria and uses nisin controlled expression system (NICE).sgRNA targets a region of pMSP3535 that codifies for RebD. RebD is a protein that initiates replication. sgRNA is under TetR repressible promoter. pMSP3535 was kindly provided to Nazarbayev University by Dr.Gary Dunny's Lab, University of Minnesota

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None