Part:BBa_K1820024
Phage inducible promoter 1997 +RBS+FspI + term
A sequence designed to provide a kill switch for Lactococcus lactis in the case of an infection by P335 species lactococcal phage Φ31.
Usage and Biology
The promoter in this sequence is A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31, containing two tandem transcription sites. Transcription from this promoter is induced after the phage genome is inserted into the bacterial cell, resulting in downstream expression in the initial stages of infection. It has been previously demonstrated that expression is successfully induced from this promoter and only occurs during phage infection. Once the promoter has been activated, the construct will produce FspI, which will kill the L. lactis cell.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 42
Illegal SapI.rc site found at 492
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