Part:BBa_K1767002

P(Lac)IQ RBS Aiia RBS LuxR ter ter luxpR RBS tetR ter ter

We designed and built a kill switch mechanism that is based on the activation of a lethal gene downstream of an inducible promoter that is activated over time.

The presented part represents the first and the second module of our designed genetic circuit. The here presented BioBrick consists of a constitutive promoter (I14032) driving expression of Aiia and LuxR followed by a Lux promoter (R0062) , driving expression of TetR.

By using this part one can control the amount of producing TetR which means that at the beginning the system will produce TetR. After a certain amount of time thatdepends on the initial concentration of AHL , the system will stop producing TetR due to the absence of AHL that interacts with LuxR to form its active conformation. The scheme below explains how this BioBrick works:

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 157
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1761