Part:BBa_K174016

Promoterless ArsR binding site

This part purely holds the binding site for ArsR protein which can be released when bound to different metals. Hence this part can be combined with any promoter to sense metals that can bind to ArsR protein. When added in front of a promoter with another metal sensor's protein's binding site, it can form an AND gate to sensitively detect specific metals.

Usage and Biology

ArsR function: Gaston Day School 2021

As(III) and As(V) have stronger effects on inducing the expression of the Ars operon, while the effects of Cd(II) and Ag(I) on the operon are relatively low. However, when the As(V) can be bound to the ArsR, this ion has to be reduced to As(III) first.

In the bacterium Cupriavidus metallidurans strain CH34, the ArsR only binds to the P/O region that belongs to its own. As a result, the arsenite and arsenate are able to induce the Ars operon. Besides those, Bi(III), Cd(II), Co(II), Pb(II), and Zn(II) are all able to induce transcription of the Ars operon.

Additional references for the ArsR promoter:

Moore, C. M., Gaballa, A., Hui, M., Ye, R. W., & Helmann, J. D. (2005). Genetic and physiological responses of bacillus subtilis to metal ion stress. Molecular Microbiology, 57(1), 27–40. https://doi.org/10.1111/j.1365-2958.2005.04642.x

Zhang, Y.-B., Monchy, S., Greenberg, B., Mergeay, M., Gang, O., Taghavi, S., & van der Lelie, D. (2009). ARSR arsenic-resistance regulatory protein from Cupriavidus metallidurans CH34. Antonie Van Leeuwenhoek, 96(2), 161–170. https://doi.org/10.1007/s10482-009-9313-z Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]