Translational_Unit

Part:BBa_K1695012

Designed by: Aruna Surendran Ramkrishnan   Group: iGEM15_CityU_HK   (2015-08-11)

Riboswitch + Bacteriophage 21 codon optimized S gene

The gene encodes the S protein (holin) from bacteriophage 21 with the anti-holin sequence removed. The gene is codon optimized and is preceded by a riboswitch for the regulation on its translation by theophylline.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 34
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None