Coding

Part:BBa_K1692003

Designed by: Daniel Kunin   Group: iGEM15_Stanford-Brown   (2015-08-03)

PAL with T7 promoter

PAL with T7 Promoter

Phenylalanine ammonia lyase (PAL) catalyzes the conversion of L-phenylalanine to trans-cinnamic acid. This part is a modification of University of British Columbia’s 2013 PAL biobrick part (BBa_K1129003) from Streptomyces maritimus. Specifically, our part contains a T7 promoter, allowing for inducible expression. See our synthesized PAL for more results.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 573
    Illegal NgoMIV site found at 676
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1272
    Illegal BsaI.rc site found at 1527


[edit]
Categories
//awards/part_collection/2015
Parameters
None