Translational_Unit

Part:BBa_K1641019

Designed by: Pai Li   Group: iGEM15_SYSU_CHINA   (2015-09-11)


Up-side-down RBS::mcherry-ssra with VloxP, Vcre recognition sites

This is a special RBS::mcherry-ssra inverted into up-side-down form, with two VloxP sites at the upstream and downstream of the gene. VloxP can be recognized by invertase Vcre and the middle mcherry can be restored through inversion. Ssra-tag in this sequence is moderately fast type (BBa_M0052) that accelerates the degradation but at a speed far less than expression.

This part can be used to construct the reporter for real-time invertase dynamics analysis.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None