Part:BBa_K1632006
fim switch[default ON](Tokyo_Tech/R0010)
The fim switch is the promoter containing repeated DNA sequence which is inverted by the Fim recombinase. Therefore, we can control the expression of the gene downstream of the fim switch by adding the Fim recombinase.
We designed the fim switch which has a J23119 promoter(BBa_K1632000) . Basically, the design of the fim switch(Tokyo_Tech) is similar to the fim switch(wild-type). The only difference is that we inserted restriction cut enzyme sites to fim switch(Tokyo_Tech). In detail, in fim switch(Tokyo_Tech) the sigma 70 promoter is exchanged to the J23119 promoter and there are two restriction enzyme cut sites inserted each in the front (SalI and BamHI) and in the back (BglII and MluI) of the promoter. Due to the insertion of the restriction enzyme cut sites, fim switch(Tokyo_Tech) has a promoter which can we exchanged with an arbitrary promoter. We actually changed J23119 promoter in the fim switch(Tokyo_Tech) to Lac promoter(BBa_R0010) as shown in the figure below.
More information
For more information, see http://2015.igem.org/Team:Tokyo_Tech/Project Our work in Tokyo_Tech 2015 wiki, http://2015.igem.org/Team:Tokyo_Tech/Experiment/ssrA_tag_degradation_assay About ssrA-tag, http://2015.igem.org/Team:Tokyo_Tech/Experiment/Overview_of_fim_inversion_system About ''fim'' inversion system
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 539
Illegal BamHI site found at 333 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |