Part:BBa_K1631024

Colicin-E3 cassette (Strong)

Colicin-E3, its immunity protein, and colicin lysis protein is constitutively expressed strong.(under BBa_?J23101) When this device works, Colicin-E3 will be released to media, and E. coli strains which does not have immunity protein will be killed.

Usage and Biology

Colicins are a cytotoxins which are released to environment and kill other related strains.

Release of colicin involves one protein; Colicin Lysis Protein. Colicin lysis protein allows colicins to be released. The mechanism how the colicin lysis protein allows colicin release has not been fully elucidated, but it is sure that this protein raise membrane permitability and cause quasilysis.[1] After Colicin released, they diffuse through the medium and bind to the receptor on the target cell membrane. Then, they are imported to the cytoplasm or cytoplasmic membrane of target cell by Tol-system or Ton-system. (If you want to know the mechanism of the Colicin import, see [1]) Colicin producing cells also express Colicin Immunity Protein in order to protect themselves from cytotoxity of colicin. Colicins have variety cytotoxity such as DNase activity, RNase activity or Pore forming across inner membrane.

Reference [1]Cascales, E., Buchanan, S. K., Duché, D., Kleanthous, C., Lloubes, R., Postle, K., ... & Cavard, D. (2007). Colicin biology. Microbiology and Molecular Biology Reviews, 71(1), 158-229.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1733
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 696
  Illegal AgeI site found at 68
  Illegal AgeI site found at 179
  Illegal AgeI site found at 448
  Illegal AgeI site found at 1714
  Illegal AgeI site found at 1819
  
• 1000
  COMPATIBLE WITH RFC[1000]