Part:BBa_K1526007

LacRS + MntH

MntH gene under control of Lac Repressor System

Gene: mntH
Organism: Escherichia Coli
Protein: MntH transporter from NRAMP family
Function: NRAMP is a membrane divalent metal-ion transporter. In addition to iron and manganese, it also transports Cd ions into the cell.
Aim: We want to use the endogenous transporter NRAMP to take up the cadmium from the environment. The cadmium will then activate pYoda and set off the phytochelatin synthesis process.
Expression: We can run assays measuring the concentration of cadmium in the environment. If MntH is active, the concentration of cadmium should decrease. Mutant strains: Information about mutant E. coli strains for MntH can be found at EchoBase website from the University of York (http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB3909).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1188
  Illegal BamHI site found at 2462
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 2358
  
• 1000
  COMPATIBLE WITH RFC[1000]