Part:BBa_K1520013

Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter

Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter. It produces rfp, luxI and luxR when there is enough luxR and AHL.

From left to right, Marker(DL10000), Prlux, Prlux-rbs-rfp, Prlux-rbs-rfp-Ter, Prlux-rbs-rfp-rbs-luxI, Prlux-rbs-rfp-rbs-luxI-Ter, Prlux-rbs-rfp-rbs-luxR-Ter, Prlux-rbs-rfp-luxR, Prlux-rbs-rfp-luxI-rbs-luR, Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter.

The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1458
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]