Reporter
Part:BBa_K1520010
Designed by: yue wu Group: iGEM14_Nanjing-China (2014-09-22)
Prlux-rbs-rfp-Ter
Prlux-rbs-rfp-Ter. It produces rfp when there is enough luxR and AHL.
From left to right, Marker(DL10000), Prlux, Prlux-rbs-rfp, Prlux-rbs-rfp-Ter, Prlux-rbs-rfp-rbs-luxI, Prlux-rbs-rfp-rbs-luxI-Ter, Prlux-rbs-rfp-rbs-luxR-Ter, Prlux-rbs-rfp-luxR, Prlux-rbs-rfp-luxI-rbs-luR, Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter.
The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//direction/forward
//function/cellsignalling/LuxR
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//function/cellsignalling/LuxR
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
control | luxR, HSL |
direction | Forward |
negative_regulators | |
o_h | |
o_l | |
positive_regulators | 1 |