Reporter
Part:BBa_K1520005
Designed by: yue wu Group: iGEM14_Nanjing-China (2014-09-22)
Plac-rbs-rfp-Ter
Plac-rbs-rfp-Ter It produces rfp when there is less lacI or most of lacI is occupied by IPTG.
From left to right Marker(DL2000), Plac, Plac-rbs-rfp, Plac-rbs-rfp-T. The substances of electrophoresis are made from PCR of plasmids with VF2 and VR as their primers.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/negative
//classic/regulatory/uncategorized
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/negative
//classic/regulatory/uncategorized
biology | |
control | IPTG, lacI |
direction | Forward |
negative_regulators | 1 |
o_h | |
o_l | |
positive_regulators |