Reporter

Part:BBa_K1520005

Designed by: yue wu   Group: iGEM14_Nanjing-China   (2014-09-22)

Plac-rbs-rfp-Ter

Plac-rbs-rfp-Ter It produces rfp when there is less lacI or most of lacI is occupied by IPTG. Rfp.jpg


K1520005.jpg

From left to right Marker(DL2000), Plac, Plac-rbs-rfp, Plac-rbs-rfp-T. The substances of electrophoresis are made from PCR of plasmids with VF2 and VR as their primers.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/negative
//classic/regulatory/uncategorized
Parameters
biology
controlIPTG, lacI
directionForward
negative_regulators1
o_h
o_l
positive_regulators