Generator

Part:BBa_K1520003

Designed by: yue wu   Group: iGEM14_Nanjing-China   (2014-09-22)

Pcons2-rbs-luxR-Ter

Pcons2-rbs-luxR-Ter. It achieves a constant expression of luxR, the AHL combining protein. Pcons2 is BBa_J23100

K1520003.jpg

From left to right, Marker(DL10000), Pcons2, Pcons2-luxR, Pconss-luxR-Ter. Primers used in PCR are VF2 and VR

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//cds/transcriptionalregulator/activator
//cds/transcriptionalregulator/repressor
//function/cellsignalling
//function/regulation/transcriptional
Parameters
directionForward
functionMixed
ligandsHSL
n/aconstitutive promoter family member
negative_regulators
positive_regulators
proteinLuxR
swissproP12746
tagNone