Generator
Part:BBa_K1520003
Designed by: yue wu Group: iGEM14_Nanjing-China (2014-09-22)
Pcons2-rbs-luxR-Ter
Pcons2-rbs-luxR-Ter. It achieves a constant expression of luxR, the AHL combining protein. Pcons2 is BBa_J23100
From left to right, Marker(DL10000), Pcons2, Pcons2-luxR, Pconss-luxR-Ter. Primers used in PCR are VF2 and VR
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds
//cds/transcriptionalregulator/activator
//cds/transcriptionalregulator/repressor
//function/cellsignalling
//function/regulation/transcriptional
//cds/transcriptionalregulator/activator
//cds/transcriptionalregulator/repressor
//function/cellsignalling
//function/regulation/transcriptional
direction | Forward |
function | Mixed |
ligands | HSL |
n/a | constitutive promoter family member |
negative_regulators | |
positive_regulators | |
protein | LuxR |
swisspro | P12746 |
tag | None |