Translational_Unit

Part:BBa_K1461318

Designed by: Shunsuke Sumi   Group: iGEM14_UT-Tokyo   (2014-09-26)

pEpCAM(mutant)-RBS-GFP-4 miR 142 3p site-d.term

This is a GFP generator. This generator is used for assay of translation inhibition by miR-142-3p on EpCAM(mutant) promoter in HL60 and cos7. This contains pEpCAM(mutant), RBS, GFP, 4 miR-142-3p binding sites and double terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None