Composite

Part:BBa_K1460008

Designed by: Eric Holmes   Group: iGEM14_Cornell   (2014-08-26)


Anderson Promoter + CBP4 + pT7 + GST-CRS5 + Ter

This composite part functionally codes for the putative lead transporter CBP4 as well as the metallothioniein CRS5 fused to glutathione-S-transferase. This composite part should act to transport lead ions into the engineered cell where they will be sequestered by the metallothionein allowing for Pb2+ removal from contaminated water.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 684
    Illegal BamHI site found at 279
    Illegal BamHI site found at 371
    Illegal BamHI site found at 2961
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2373


[edit]
Categories
//binding/metal
Parameters
None