Composite

Part:BBa_K1460005

Designed by: Eric Holmes   Group: iGEM14_Cornell   (2014-08-26)


Anderson Promoter + RBS + CBP4

This is a functional coding sequence for the putative lead and nickel transport protein CBP4 from Nicotiana tabacum. It has been found that expression of this protein leads to increased nickel tolerance and lead intolerance.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 684
    Illegal BamHI site found at 279
    Illegal BamHI site found at 371
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//binding/metal
Parameters
None