Part:BBa_K1427000
merRNA, c-di-GMP aptamer
The merRNA (massively expressed regulatory RNA) is a long, non-coding RNA transcript found in Bdellovibrio bacteriovorus which was described by Karnuker et al 2013. The 445 nt transcript contains a cyclic-di-GMP riboswitch sequence and is believed to mediate a change from growth phase to attack phase in Bdellovibrio by sequestering the second messenger c-di-GMP. When expressed constitutively under a T7 promoter in K12 E. coli, the merRNA increased biofilm formation. This is thought to be the result of the merRNA binding to and sequestering c-di-GMP molecules. This causes an increase in motility and flagella expression. The additional flagella adhere to surfaces and other cells, thereby forming a robust biofilm. Teams can insert this part directly downstream of a promoter sequence to cause stronger biofilm formation.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 398
Illegal NheI site found at 426 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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