Translational_Unit

Part:BBa_K1402015

Designed by: Chen Xi   Group: iGEM14_NJU-QIBEBT   (2014-10-04)

RBS-BTE-Terminator

Acyl carrier protein (ACP) thioesterase catalyzes the hydrolysis of acyl-ACP into fatty acid and ACP. This reaction removes the coupling between fatty acid biosynthesis and phospholipid biosyntheis, which is a key to obtaining free fatty acids. This thioesterase BTE can produce fatty acids with 12~14 carbon chain length. In previous reserches, thioesterase gene BTE can be expressed in E.coli BL21(DE3) and enhance the synthesis of free fatty acids.This thioesterase can not express itself.The RBS is in front of the BTE coding sequence and T7 terminator is after BTE. You can design your own promoter for this part, but use this part in E.coli BL21(DE3) only or you’d better delete the T7 terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 496
    Illegal BglII site found at 636
    Illegal BamHI site found at 85
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1045


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Categories
Parameters
None