Part:BBa_K1400002
PGAL Modified native pGAL
This is the native pGAL1 promoter in S. cerevisiae with its Mig1 sites removed. This is a single input promoter that has four upstream activating sequences (UAS), which are GAL4 binding elements. The Mig1 sites are binding sites for proteins responsible for transcriptional repression of downstream genes to this and other promoters in S. cerevisiae in the presence of glucose. The removal of the Mig1 sites allows transcriptional activation of the promoter in the presence of glucose in the cellular growth medium.
Figure 1: Characterization of pGAL via dual drug induction. pGAL has no repressing sites and 4 activating GAL4 sites, so increasing estradiol increases activation while aTc has no effect beyond auto-fluorescence. The fusion protein, GEV (GAL4 binding domain-human estrogen receptor-VP16 activator domain), is the activator and is expressed with the weak constitutive promoter, pMRP7.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 76
- 1000COMPATIBLE WITH RFC[1000]
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