Part:BBa_K1398000

XenB (Xenobiotic Reductase B)

Xenobiotic Reductase B, created for use as a Trinitrotoluene and Nitroglycerine degrading protein.

A monomeric flavin that can reduce certain nitro- groups to nitrate-. Increases the resistance of organisms to the toxic effects of nitrocompounds.

This sequence encodes for a protein (with an attached His (x6) Tag to allow for purification) and nothing else. It therefore requires regulatory sequences (promoter/RBS/terminator) to be added for expression.

The protein has been codon-optimised for expression in E. coli using DNA2.0 GeneGPS Technology, and synthesized as a single operon by DNA2.0 into the pACYCDuet-1 expression vector (Novagen) multiple cloning site (MCS) 1. More information can be found here: https://www.dna20.com/services/genegps.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]