Coding

Part:BBa_K1321111

Designed by: Xenia Spencer-Milnes   Group: iGEM14_Imperial   (2014-10-09)

Phytochelatin (PC) EC20 fused with CBDclos with LacI promoter

A LacI-promoter expression construct of Synthetic phytochelatin EC20 (metal binding peptide) fused N-terminally to CBDclos (a cellulose-binding domain).

This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below: IC14-PC-part-table.PNG

Note that the stop codon plus 6 bp at the end of the sequence are included the RFC25 suffix which is not shown. The prefix to this part is RFC10 format.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 230
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//binding/cellulose
Parameters
None