Conjugation
oriTr/LacZ

Part:BBa_K125350

Designed by: Margaret Ruzicka   Group: iGEM08_Hawaii   (2008-10-27)


oriTr with lac promoter and lacZ

This part was constructed to compare the oriTr, which is currently available in the registry to oriT which was recently designed to perform the same function. The oriTr was added to LacZ part so that conjugation could be monitored by blue/white selection.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 725
    Illegal NgoMIV site found at 735
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 627


[edit]
Categories
//DNA/conjugation
//classic/reporter
//function/conjugation
Parameters
None