RNA

Part:BBa_K1212001

Designed by: Aura Ferreiro   Group: iGEM13_UC_Davis   (2013-09-16)

Theophylline Riboswitch (Clone E)


Riboswitch Clone E encodes a RNA secondary structure that acts as a translational activator. In the presence of theophylline, the theophylline aptamer binds it inducer and causes a conformational change in the RNA structure that makes available the previously sequestered RBS, thus allowing translation of the downstream sequence. This riboswitch achieves a 60-fold activation ratio in 2mM theophylline as reported by Topp et al. See figures below. [1]



We, the UC Davis iGEM 2013 team, used this riboswitch to control the expression of TAL repressors in our RiboTAL constructs, please see parts K1212014 and K1212015 for further characterization.


References

[1] S. Topp, C. M. K. Reynoso, J. C. Seeliger, I. S. Goldlust, S. K. Desai, D. Murat, et al., "Synthetic Riboswitches That Induce Gene Expression in Diverse Bacterial Species (vol 76, pg 7881, 2010)," Applied and Environmental Microbiology, vol. 77, pp. 2199-2199, Mar 2011.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None