RNA

Part:BBa_K1212000

Designed by: Aura Ferreiro   Group: iGEM13_UC_Davis   (2013-09-16)

Theophylline Riboswitch (Clone 8.1*)


Theophylline Riboswitch Clone 8.1* encodes a RNA secondary structure that acts as a translational activator. In the presence of theophylline, the theophylline aptamer binds it inducer and causes a conformational change in the RNA structure that makes available the previously sequestered RBS, thus allowing translation of the downstream sequence. This riboswitch provides a 59 - fold activation ratio in 1mM theophylline as reported by Gallivan and Lynch in the figures below [1].


We, the UC Davis iGEM 2013 team, used this riboswitch to control the expression of TAL repressors in our RiboTAL constructs, please see parts K1212011 and K1212012 for further characterization.

References

[1] S. A. Lynch and J. P. Gallivan, "A flow cytometry-based screen for synthetic riboswitches," Nucleic Acids Research, vol. 37, pp. 184-192, Jan 2009.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 42
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 42
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 42
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 42
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None