Translational_Unit

Part:BBa_K1202110

Designed by: safa tapan   Group: iGEM13_ATOMS-Turkiye   (2013-09-24)

AI-2 Generator (LuxS-Pfs)

Alternative to our NanoFactory design, we plan to use our E.coli as a nano scale cancer searching device by presenting NanoFactory Binding Zone on the outer membrane and, in the case of detection, signal transmitter by producing AI-2 into the media. This part works as NanoFactory Enzyme Zone in bacteria by transforming intracellular SAH into AI-2.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 300
    Illegal AgeI site found at 708
    Illegal AgeI site found at 825
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None