Translational_Unit

Part:BBa_K118004

Designed by: Andrew Hall   Group: iGEM08_Edinburgh   (2008-08-08)

rbs+dxs (1-deoxyxylulose-5-phosphate synthase)

This is the coding sequence of dxs from Escherichia coli JM109 with added ribosome binding site (rbs). It encodes 1-deoxyxylulose-5-phosphate synthase, thiamine-requiring, which catalyses the first step in the biosynthesis of terpenoids. Overexpression of dxs has been reported to increase yields of carotenoids and other terpenoids (Kang, M.J., Lee, Y.M., Yoon, S.H., Kim, J.H., Ock, S.W., Jung, K.H., Shin, Y.C., Keasling, J.D., and Kim, S.W. 2005. Identification of genes affecting lycopene accumulation in Escherichia coli using a shot-gun method. Biotechnology and Bioengineering 91, 636-642).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 746


[edit]
Categories
//classic/generator/rc
//function/biosynthesis/isoprenoid
Parameters
None