Part:BBa_K1159314

TMD with N-terminal Signal Peptide of SERK Receptor from ''P. patens'' in RFC[25] N-Part

This part codes for the Transmembrane Domain with N-terminal Signal Peptide of SERK Receptor from P. patens in RFC[25]. This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Protein data table for BioBrick BBa_ automatically created by the BioBrick-AutoAnnotator version 1.0

Nucleotide sequence in RFC 25 N-Part using the stop codon in the suffix, so ACCGGT was added (in italics) to the 3' end: (underlined part encodes the protein)
AACAATGCCTGGT ... ATTGAGGCTACCGGT
ORF from nucleotide position 5 to 298 (excluding stop-codon)

Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below)

1	MPGEVAWWRPLFLIALMPIGVLSNAEGDALNTTGPGQPPFPPPPPFTPPPPQTPNGASGENSTGAIAGGVAAGAALLFAAPAIGFAWWRRRRPIEATG*

Sequence features: (with their position in the amino acid sequence, see the list of supported features)

RFC25 scar (shown in bold):

33 to 34

Amino acid composition:

Ala (A)	16 (16.3%)
Arg (R)	5 (5.1%)
Asn (N)	4 (4.1%)
Asp (D)	1 (1.0%)

Cys (C)	0 (0.0%)
Gln (Q)	2 (2.0%)
Glu (E)	4 (4.1%)
Gly (G)	13 (13.3%)

His (H)	0 (0.0%)
Ile (I)	5 (5.1%)
Leu (L)	7 (7.1%)
Lys (K)	0 (0.0%)

Met (M)	2 (2.0%)
Phe (F)	5 (5.1%)
Pro (P)	18 (18.4%)
Ser (S)	3 (3.1%)

Thr (T)	6 (6.1%)
Trp (W)	4 (4.1%)
Tyr (Y)	0 (0.0%)
Val (V)	3 (3.1%)

Amino acid counting

	Total number:	98
	Positively charged (Arg+Lys):	5 (5.1%)
	Negatively charged (Asp+Glu):	5 (5.1%)
	Aromatic (Phe+His+Try+Tyr):	9 (9.2%)

Biochemical parameters

	Atomic composition:	C₄₆₃H₆₉₉N₁₂₃O₁₂₄S₂
	Molecular mass [Da]:	10036.5
	Theoretical pI:	6.07
	Extinction coefficient at 280 nm [M^-1 cm^-1]:	22000 / 22000 (all Cys red/ox)

Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges

Codon usage

	Organism:	E. coli	B. subtilis	S. cerevisiae	A. thaliana	P. patens	Mammals
	Codon quality (CAI):	good (0.70)	good (0.72)	good (0.77)	excellent (0.92)	excellent (0.90)	good (0.75)

Alignments (obtained from PredictProtein.org)

	SwissProt:	Q94F62 (65% identity on 20 AAs), Q9SKG5 (45% identity on 67 AAs)
	TrEML:	A9RY79 (80% identity on 81 AAs), B9FZB8 (77% identity on 36 AAs)
	PDB:	-

Predictions (obtained from PredictProtein.org)

Subcellular Localization (reliability in brackets)

	Archaea:	secreted (63%)
	Bacteria:	secreted (45%)
	Eukarya:	mitochondriamembrane (41%)

Gene Ontology (reliability in brackets)

	Molecular Function Ontology:	-
	Biological Process Ontology:	-

Predicted features:

	Disulfid bridges:	-
	Transmembrane helices:	67 to 84 going inwards

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