Part:BBa_K1159204

Secretory SpyCatcher (SERK-SigP_SpyCatcher) in RFC[25] N-Part

This part encodes the SpyCatcher BBa_K1159200 with a N-terminal signal peptide for secretion (SERK receptor signal peptide from Physcomitrella patens). This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Protein data table for BioBrick BBa_ automatically created by the BioBrick-AutoAnnotator version 1.0

Nucleotide sequence in RFC 25 N-Part using the stop codon in the suffix, so ACCGGT was added (in italics) to the 3' end: (underlined part encodes the protein)
AACAATGCCTGGT ... GCTCATATTACCGGT
ORF from nucleotide position 5 to 451 (excluding stop-codon)

Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below)

1
101

MPGEVAWWRPLFLIALMPIGVLSNAEGDALNTTGVDTLSGLSSEQGQSGDMTIEEDSATHIKFSKRDEDGKELAGATMELRDSSGKTISTWISDGQVKDF
YLYPGKYTFVETAAPDGYEVATAITFTVNEQGQVTVNGKATKGDAHITG*

Sequence features: (with their position in the amino acid sequence, see the list of supported features)

RFC25 scar (shown in bold):

33 to 34

Amino acid composition:

Ala (A)	13 (8.7%)
Arg (R)	3 (2.0%)
Asn (N)	4 (2.7%)
Asp (D)	11 (7.4%)

Cys (C)	0 (0.0%)
Gln (Q)	5 (3.4%)
Glu (E)	11 (7.4%)
Gly (G)	17 (11.4%)

His (H)	2 (1.3%)
Ile (I)	8 (5.4%)
Leu (L)	10 (6.7%)
Lys (K)	8 (5.4%)

Met (M)	4 (2.7%)
Phe (F)	5 (3.4%)
Pro (P)	5 (3.4%)
Ser (S)	11 (7.4%)

Thr (T)	16 (10.7%)
Trp (W)	3 (2.0%)
Tyr (Y)	4 (2.7%)
Val (V)	9 (6.0%)

Amino acid counting

	Total number:	149
	Positively charged (Arg+Lys):	11 (7.4%)
	Negatively charged (Asp+Glu):	22 (14.8%)
	Aromatic (Phe+His+Try+Tyr):	14 (9.4%)

Biochemical parameters

	Atomic composition:	C₇₀₀H₁₀₈₈N₁₈₂O₂₃₄S₄
	Molecular mass [Da]:	15925.7
	Theoretical pI:	4.42
	Extinction coefficient at 280 nm [M^-1 cm^-1]:	22460 / 22460 (all Cys red/ox)

Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges

Codon usage

	Organism:	E. coli	B. subtilis	S. cerevisiae	A. thaliana	P. patens	Mammals
	Codon quality (CAI):	good (0.79)	good (0.76)	good (0.76)	excellent (0.82)	good (0.80)	good (0.68)

Alignments (obtained from PredictProtein.org)

	SwissProt:	-
	TrEML:	Q879P6 (88% identity on 129 AAs), Q8G9G1 (88% identity on 129 AAs)
	PDB:	2x5p (92% identity on 99 AAs)

Predictions (obtained from PredictProtein.org)

Subcellular Localization (reliability in brackets)

	Archaea:	secreted (100%)
	Bacteria:	cytosol (28%)
	Eukarya:	secreted (29%)

Gene Ontology (reliability in brackets)

	Molecular Function Ontology:	-
	Biological Process Ontology:	-

Predicted features:

	Disulfid bridges:	-
	Transmembrane helices:	-

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