Coding

Part:BBa_K115050

Designed by: Ruud Jorna   Group: iGEM08_TUDelft   (2008-07-15)


A-coA -> AA-coA

This is the first enzyme in the mevalonate pathway we want to introduce into E. coli. It is needed to turn acetyl-coA into acetoacetyl-coA. The enzyme itself originates from E. coli and is called AtoB (AE014075). An alternative to this would be ERG10 from S. Cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 652
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 652
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 652
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 652
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 652
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/biosynthesis/isoprenoid
Parameters
None