RNA

Part:BBa_K1126007

Designed by: Keita Kinose   Group: iGEM13_Kyoto   (2013-09-13)

Fusion6 antisense

Fusion6 antisense is the functional RNA which regulates the transcription of downstream gene of Fusion6 attenuator (BBa_K1126004).

In the absence of antisense RNA, secondary structure of Fusion3m2 attenuator locus does not affect transcription. However, when mRNA of this locus is partly coupled complementary with mRNA of Fusion6 antisense, this locus is folded into Rho-independent terminator and terminates the transcription right before the gene. Downstream gene is somewhat silenced by this mechanism (the efficiency is shown to be 44% in Escherichia coli).

This mechanism is originally found in the regulation system of plasmid pT181 in gram negative bacteria Staphylococcus aureus, and Fusion6 antisence is made by arranging the sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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