Translational_Unit

Part:BBa_K1122678

Designed by: Aleksandra Lewicka, Jan Lyczakowski   Group: iGEM13_Edinburgh   (2013-08-30)


pSpac - LacZ - pdc- adhB

This translational unit enables expression of pyruvate decarboxylase (pdc) and alcohol dehydrogenase B (adhB) in Bacillus subtilis using xylose induction. Production of pdc and adhB enzymes allows for conversion of pyruvate to ethanol via an acetaldehyde intermediate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 899
    Illegal AgeI site found at 2105
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None