Part:BBa_K1093017

mCherry C-terminal

This is the C-terminus of mCherry, that can be used in Bimolecular Fluorescence Complementation system (BiFC). Split between amino acids 159 and 160. Emission/excitation maxima are 587/610 nm. Known to have short maturation time and can be potentially observed with naked eye. Alongside BBa_K1093016 this part can be used for investigating protein-protein interactions.

References

1: Fan JY, Cui ZQ, Wei HP, Zhang ZP, Zhou YF, Wang YP, Zhang XE. Split mCherry as a new red bimolecular fluorescence complementation system for visualizing protein-protein interactions in living cells. Biochem Biophys Res Commun. 2008 Feb 29;367(1):47-53. Epub 2007 Dec 26. PubMed PMID: 18158915.

2: Kodama Y, Hu CD. Bimolecular fluorescence complementation (BiFC): a 5-year update and future perspectives. Biotechniques. 2012 Nov;53(5):285-98. doi: 10.2144/000113943. Review. PubMed PMID: 23148879.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]