Translational_Unit

Part:BBa_K1087000

Designed by: Tong Xu   Group: iGEM13_SCU_China   (2013-08-28)


RBS-cre-ssrA(LAA)tag

This is a cre coding region with RBS and a ssrA(LAA) tag,which when fused to the C-terminal of proteins, will make the protein susceptible to very fast degradation through SspB-mediated binding to the ClpX protease as well as ClpA protease.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 393
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 79
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None