Part:BBa_K103013

linker fused to omega fragment of TEM beta-lactamase

Usage and Biology omega fragment of the split enzyme TEM β-lactamase β-lactamase activity can be recovered when halves are brought in close proximity by e.g. fusion to interacting domains [http://www.ncbi.nlm.nih.gov/pubmed/17005637?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum] works with second half of β-lactamase (part BBa_K103009) contains linker module that allows proper binding of fusion partners This part uses our NdeI/SacI/BamHI fusion cloning substandard

This protein structure was predicted using threading and protein fold prediction and may differ from actual one.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 190