Device

Part:BBa_K091203

Designed by: Todd Eckdahl   Group: iGEM08_Davidson-Missouri_Western   (2008-09-04)


pBAD-RBS-LuxR-RBS-LacI(I12)-TT

This expression casette constitutively produces the LuxR regulatory protein that can respond to the quorum sensing molecule 3OC6. It also produces a LacI repressor with a mutation at position 12 that results in an isoleucine. The mutant repressor has been shown to have an increased affinity for its operator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/cellsignalling
//function/regulation/transcriptional
Parameters
None