Part:BBa_J70303

Test Cm resistance plasmid for Mesoplasma florum

Assembly of pSCI2 plasmid origin and cat cassette into E. coli plasmid pSB1T3.

Usage and Biology

MIC in E. coli DH10B cells is 1 ug/ml of chloramphenicol. This is near background; the cassette appears not to function in E. coli.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 372