Part:BBa_J70040

A BioScaffold Part (Uses AarI, MabI) for Library Vector Preparation (see Part Design page)

When placed in a BioBrick assembly and cut with the restriction enzyme AarI, this part will direct the restriction enzyme to cut within the BioBrick scar still in testing, uses AarI (2), MabI(2), XmaI (1) * XmaI does not work in vector for BioScaffold part manipulations See part design page or BBFRFC15, for details about use

Oligos were ordered 3/18/09:

 AATTCGCGGCCGCTTCTAGAGGCAGGTGGACACCTAGGGTCCCGGGAGCACCAGGTCCCACCTGCATA (CATG)
                      gcaggtggacacctagggtcccgggagcaccaggtcccacctgca
     G                                                          |  at  gatc
gaattcgcggccgcttctagag                                             ta  ctagtagcggccgctgcag
BioBricks Prefix                                                                BioBricks Suffix

Oligos for insertion into a EcoRI/SpeI cut vector such as pSB2K4:

Forward 40.1.F-1: AAT TCG CGG CCG CTT CTA GAG
Forward 40.1.F-2: GCA GGT GGA CAC CTA GGG TCC CGG GAG CAC CAG GTC CCA CCT GCA TA
Reverse 40.1.R-1: CTA GTA TGC AGG TGG GAC CTG GTG CTC CCG GG
Reverse 40.1.R-2: ACC CTA GGT GTC CAC CTG CCT CTA GAA GCG GCC GCG

Prototype Oligos (DO NOT USE, Only use for designing the correct oligos.) 
(these oligos are wrong--do not use them as they do not include MabI site--they are from J70034, 
however, they show appropriate places to cut the part into oligos )
Forward 1: AATTCGCGGCCGCTTCTAGAG
(this oligo is wrong--does not include MabI site) 
Forward 2: GCAGGTGGACAAGAGGAGTCCCGGGAGCTGGAACTCCCACCTGCATA
Reverse 1: ACTCCTCTTGTCCACCTGCCTCTAGAAGCGGCCGCG
Reverse 2: CTAGTATGCAGGTGGGAGTTCCAGCTCCCGGG

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]