Regulatory

Part:BBa_J64067

Designed by: Jeffrey J Tabor   Group: Voigt Lab   (2007-12-12)

LuxR+3OC6HSL independent R0065

This part is derived from R0065, a hybrid LuxR+3OC6HSL activated, CI repressed promoter. To eliminate LuxR+3OC6HSL dependence, the weak -35 hexamer of R0065 was changed to the consensus TTGACA sequence. Then, to increase the consitutive transcription rate, two modifications were made. First, a single base was been added to the region between the -35 and -10 elements, bringing that region to the optimal length of 17bp. Second, an 'extended -10' sequence was added 1 base pair upstream of the -10 hexamer. The two CI operators OR1 and OR2 were left unchanged. Taken together, this should be a strong CI-repressible promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/negative
//function/cellsignalling/LuxR
Parameters
negative_regulators1
positive_regulators