Part:BBa_J58104

Fusion protein Trg-EnvZ for signal transduction

This part implements a synthetic two-component signal transduction pathway. We created a chimeric coding region part for a protein in which Trg (chemoreceptor) contributed its periplasmic and transmembrane domains (as well as a short cytoplasmic segment) and EnvZ (osmosensor) contributed its cytoplasmic kinase/phosphatase domain.

By Valencia iGEM 2006

A. Aparici (2), MC. Aroca (2), J. Carrera (1), C. Edo (1,2), G. Fuertes (2), D. Jimenez (2), C. Mata (2), JV. Medrano (2), A. Montagud (2), C. Navarrete (2), E. Navarro (1), G. Rodrigo (1), M. Baguena (1), P. Tortosa (3), P. Fernandez-de-Cordoba (1), A. Ferrando (2), J. Salgado (2), J. Urchueguia (1), A. Jaramillo (3).

1 Universidad Politecnica de Valencia, Spain

2 Universitat de Valencia, Spain

3 Ecole Polytechnique, France

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 348
  
• 1000
  COMPATIBLE WITH RFC[1000]