Reporter

Part:BBa_J44010

Designed by: Todd Eckdahl   Group: iGEM06_Missouri   (2006-10-26)

HixC-pBAD-HixC-RBS-TetF-HixC

Construct for use in detecting the activity of Hin recombinase system. pBAD promoter is flanked by HixC recombination sites and may be flipped from the right to the left orientation. Tet resistance gene with upsteam RBS are also flanked by HixC sites and may be flipped from forward to reverse orientation. Cloned in pSB1A2 - readthrough transcription detected.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 159
    Illegal NheI site found at 371
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 99
    Illegal BamHI site found at 517
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 543
    Illegal NgoMIV site found at 911
    Illegal NgoMIV site found at 1071
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/recombination/hin
Parameters
None